ACTR3

Cilia effects upon perturbation of ACTR3

Cilia number / % ciliated:

Incrased cilia number

Loss-of-function effect:

Longer cilia

Ciliogenesis screen results (5 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: Increased Signaling (Negative Regulator) PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
• Kim et al. 2010 (siRNA) [siRNA]: Longer Cilia (Area per Cilia z=5.04) PMID:20393562

Subcellular localization

basal body, cilia, cytosol

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Actin & cytoskeleton regulation
• Viral interactions
• Signaling (Hedgehog, GPCRs, ion channels)

Function

ACTR3 is necessary for nucleating actin polymerization at filament branches, caused a significant increase in cilium length and also facilitated ciliogenesis independently of serum starvation (20393563). Arp3 of the Arp2/3 complex and actin nucleator N-WASp localized to the basal body at the base of the primary cilium in mDCs (29945904)

Model organism evidence