AKAP9

Cilia effects upon perturbation of AKAP9

Ciliogenesis screen results (4 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-4.73) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
• Pusapati et al. 2018 (CRISPR) [CRISPR]: Negative Regulator (Hh signaling, pos_rank=772, lfc=0.52) PMID:30270045

Phenotypes

Mouse phenotype:

increased circulating alkaline phosphatase level, decreased circulating cholesterol level, increased circulating amylase level, decreased total body fat amount, increased lean body mass, decreased body weight, increased bone mineral density, decreased circulating ldl cholesterol level, preweaning lethality, incomplete penetrance, abnormal head morphology, decreased circulating serum albumin level, increased bone mineral content, decreased circulating hdl cholesterol level

Subcellular localization

basal body, centrosome, cytosol, microtubules

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• Protein processing & maturation
• Signaling (Hedgehog, GPCRs, ion channels)
• Cardiac & muscle development
• Muscle contraction & physiology

Function

PKARII was colocalized with AKAP9 to centrosome-Golgi in all the wild type cells examined (PKA-mediated Gli2 and Gli3 phosphorylation is inhibited by Hedgehog sig ling in cilia and reduced in Talpid3 mutant).