APC

Cilia effects upon perturbation of APC

Cilia number / % ciliated:

No effect

Loss-of-function effect:

Longer cilia

Ciliogenesis screen results (5 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-5.17) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
• Pusapati et al. 2018 (CRISPR) [CRISPR]: Negative Regulator (Hh signaling, pos_rank=730, lfc=0.71) PMID:30270045

Subcellular localization

basal body, centrosome, cytosol, microtubules, nucleus

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• T cell biology
• Signaling (Hedgehog, GPCRs, ion channels)

Function

Inhibition of APC/C function by knockdown of the A PC2 subunit disrupts the polarity of motile cilia and alters the directio lity of the fluid movement along the epidermis of the Xenopus embryo (19805045).APC–Cdc20 complex acts as a negative regulator of ciliary length and is required for proper ciliary resorption when quiescent cells re-enter the cell cycle.Inhibition of APC2 or Cdc20 caused longer cilia in quiescent cells.Overexpression of Cdc20 abolishes ciliogenesis entirely (PMID: 25139956).

Model organism evidence