APOE

Cilia effects upon perturbation of APOE

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.78) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

increased circulating triglyceride level, increased circulating cholesterol level, increased circulating ldl cholesterol level, decreased circulating glucose level, increased circulating alkaline phosphatase level, increased total body fat amount, decreased circulating serum albumin level, abnormal glucose homeostasis, abnormal tooth morphology

Mouse ciliopathy phenotype:

increased circulating bilirubin level

Subcellular localization

cilia associated gene, nucleus

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Viral interactions
• Cell migration & adhesion
• Signaling (Hedgehog, GPCRs, ion channels)
• Cardiac & muscle development

Function

Removing endothelial cilia increased atherosclerosis in Apoe−/− mice fed a high‐fat, high‐cholesterol diet, indicating that cilia protect against atherosclerosis( 26769565).

Model organism evidence