ARF6

Subcellular localization

Cytosol

References: PMID:40503937, PMID:31694916, PMID:33483367, PMID:41858616, PMID:38530365

Functional category

• Non-motile cilium / primary cilium
• Trafficking (BBSome
• small GTPases
• vesicular transport
• ATPases)
• Ciliary assembly/disassembly

Function

Using immunoblotting to specifically examine the expression levels of ADP-ribosylation factor 6 (ARF6) and EPS8-like 2 (EPS8L2) in EVs, we found that EXOC5 KD increases ARF6 levels and decreases EPS8L2 levels, and that EXOC5 overexpression increases EPS8L2. Knockout of intraflagellar transport 88 (IFT88) confirmed that the changes in EV number/content were due to cilia loss: similar to EXOC5, the IFT88 loss resulted in very short or absent cilia, decreased EV numbers, increased EV ARF6 levels, and decreased Eps8L2 levels compared with IFT88-rescued EVs. Compared with control animals, urine from proximal tubule-specific EXOC5-KO mice contained fewer EVs and had increased ARF6 levels(31694916). Merlin-ARF6 complex regulates SMO traffic to the primary cilium in RAB11+ endosomes(40503937). Arf6 is required for ciliogenesis and its active form rescues ciliogenesis impaired by EFA6A depletion(33483367).