ARHGAP35

Rho GTPase activating protein 35

Tier: Gold Standard Ciliary Genes

Ensembl:
ENSG00000160007
UniProt:
Q9NRY4
OMIM:
605277
Synonyms:
GRF-1, GRLF1, KIAA1722, P190A, P190ARHOGAP, P190RHOGAP

Cilia effects upon perturbation of ARHGAP35

Cilia number / % ciliated:
Decrease
Loss-of-function effect:
Decrease

Ciliogenesis screen results (2 screens)

  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-3.74) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680

Subcellular localization

basal body

References: PMID:32663194

Functional category

  • Ciliary assembly/disassembly
  • Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
  • Actin & cytoskeleton regulation
  • Neurogenesis & migration
  • Signaling (Hedgehog, GPCRs, ion channels)
  • Axon guidance & growth
  • Cilia length regulation

Function

Using cilia length as a phenotypic readout for centrosomal RhoA activity, ARHGAP5, -29, and -35 as essential regulators of ciliation in normal human renal tubular cells and in PKD1-null cells, centrosomal ARHGAP35 decreased(32663194). ARHGAP35 (p190A RhoGAP) is recruited by FUZ/FUZZY to the basal body where it restricts actin polymerization via RhoA inhibition to allow ciliogenesis. Loss increases actin at basal body → impaired ciliogenesis. Genetic interactions with FUZ confirmed in mice.