ARHGAP5

Rho GTPase activating protein 5

Ensembl:
ENSG00000100852
UniProt:
Q13017
OMIM:
602680
Synonyms:
GFI2, P190-B, P190BRHOGAP, RHOGAP5

Cilia effects upon perturbation of ARHGAP5

Cilia number / % ciliated:
Decreased cilia number
Loss-of-function effect:
Shorter cilia

Ciliogenesis screen results (3 screens)

  • Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, casTLE effect=-0.97) PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Subcellular localization

basal body, cytosol

Functional category

  • Ciliary assembly/disassembly
  • Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
  • Cell migration & adhesion
  • Cilia length regulation

Function

Using cilia length as a phenotypic readout for centrosomal RhoA activity, ARHGAP5, -29, and -35 are essential regulators of ciliation in normal human re l tubular cells. The centrosome retention of ARHGAP5 and -29 are determined by their interaction with other proteins apart from PC1(32663194).

Model organism evidence

Mus musculus (1 reference)

Using cilia length as a phenotypic readout for centrosomal RhoA activity, we identified ARHGAP5, -29, and -35 as essential regulators of ciliation in normal human renal tubular cells.

PMIDs: 32663194