ATG7

Cilia effects upon perturbation of ATG7

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Shorter cilia

Ciliogenesis screen results (4 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-3.32) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

increased circulating serum albumin level, no spontaneous movement, abnormal mammary gland morphology, abnormal embryo size, increased circulating total protein level, preweaning lethality, incomplete penetrance, abnormal skin morphology

Mouse ciliopathy phenotype:

abnormal heart morphology, enlarged heart, abnormal reti morphology

Human ciliopathy phenotype:

spinocerebellar ataxia, autosomal recessive 31

Subcellular localization

cytosol, flagella

Functional category

• Ciliary assembly/disassembly
• Reproduction & sperm
• Signaling (Hedgehog, GPCRs, ion channels)

Function

Atg7 is required for spermatozoa flagella biogenesis and cytoplasm removal during spermiogenesis (27310465) localized to flagella (25613900). In atg7-KO mice, cilium length and frequency in kidney proximal tubular cells were significantly reduced compared to those of wild-type tissues (PMID: 25906314).

Model organism evidence