BECN1

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Hyper-ciliogenesis (z=2.41) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

increased total body fat amount; increased circulating bilirubin level; decreased circulating HDL cholesterol level; increased leukocyte cell number; preweaning lethality; complete penetrance; decreased lean body mass; increased startle reflex

Subcellular localization

cytosol, endoplasmic reticulum, nuclear bodies

References: PMID:25906314

Functional category

• Non-motile cilium / primary cilium

Function

Autophagy is required for dismantling the primary cilium during cell cycle re-entry. BECN1 peptide lengthened primary cilia after 4 h of treatment, compared to the scrambled peptide-treated controls. Surprisingly, IFT88 was downregulated by BECN1 peptide (Fig. 3C), suggesting that ciliary factor(s) other than IFT88 may be responsible for the observed ciliary lengthening. Obviously, all 3 methods increased LC3B-II levels. SQSTM1 was degraded in RKRB and BECN1 peptide-treated cells(PMID: 25906314).