BECN1

beclin 1

Ensembl:
ENSG00000126581
UniProt:
Q14457
OMIM:
604378
Synonyms:
ATG6, VPS30

Cilia effects upon perturbation of BECN1

Ciliogenesis screen results (3 screens)

  • Wheway et al. 2015 (siRNA) [siRNA]: Hyper-ciliogenesis (z=2.41) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:
increased total body fat amount; increased circulating bilirubin level; decreased circulating HDL cholesterol level; increased leukocyte cell number; preweaning lethality; complete penetrance; decreased lean body mass; increased startle reflex

Subcellular localization

cilia associated gene, cytosol, endoplasmic reticulum, nuclear bodies

Functional category

  • Non-motile cilium / primary cilium

Function

Autophagy is required for dismantling the primary cilium during cell cycle re-entry. BECN1 peptide lengthened primary cilia after 4 h of treatment, compared to the scrambled peptide-treated controls. Surprisingly, IFT88 was downregulated by BECN1 peptide (Fig. 3C), suggesting that ciliary factor(s) other than IFT88 may be responsible for the observed ciliary lengthening. Obviously, all 3 methods increased LC3B-II levels. SQSTM1 was degraded in RKRB and BECN1 peptide-treated cells(PMID: 25906314).

Model organism evidence

Mus musculus (2 references)

Based on observations that increased morphological and biochemical indicators of autophagy correlate with cilia shortening in our models, we posited that autophagy might regulate cilia length in response to CS in the lung.

Exposure to CS reduced cilia length and induced autophagy in vivo and in differentiated mouse tracheal epithelial cells (MTECs).

PMIDs: 24401596, 24200693