CCDC15

Cilia effects upon perturbation of CCDC15

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Shorter cilia

Ciliogenesis screen results (2 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680

Subcellular localization

basal body, centrosome

Functional category

• Ciliary assembly/disassembly
• Signaling (Hedgehog, GPCRs, ion channels)

Function

CCDC15 depletion affects centriole length and integrity, leading to defective cilium formation, mainte nce, and response to Hedgehog sig ling(37934472). While control cells ciliated at 70.1% ± 6.4, CCDC15-depleted cells ciliated at 45.6% ± 0.8 . As quantified from immunofluorescence images and assessed by U-ExM, the cilia that formed in CCDC15-depleted cells were significantly shorter in length (37934472).