?

Subcellular localization

basal body

Functional category

• Motile cilium & axoneme
• Non-motile cilium / primary cilium
• Ciliary assembly/disassembly
• Cilia–cytoskeleton/adhesion links

Function

To validate the novel candidates, four uncharacterized proteins—Coiled-Coil Domain-Containing 30(Ccdc30), Membrane Occupation and Recognition Nexus-motif protein 2 (Morn2), Migration and Invasion Inhibitory Protein (Miip) and WD Repeat Domain 88 (Wdr88) were selected. Whole-mount in situ hybridization confirmed that all four genes were specifically expressed in epidermal MCCs, with miip showing earlier expression at stages 17-18, just before MCC ciliation (Figure 2A). Ccdc30 showed minimal overlap with Cep164 and appeared as a larger, more distally positioned ring structure as compared to Cep164 was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. (Figure S1A, Video S1). Furthermore, Cep164 accumulation was preserved in the majority of centrioles in Ccdc30 morphants (Figure S1B), indicating that distal appendage formation and centriole maturation are likely unaffected by Ccdc30 depletion. However, a marked reduction in centriole number was observed in Ccdc30 morphants (Figure S1B, Figure 4J and 4K), a defect that was fully rescued by co-injection of morpholino-resistant Ccdc30 mRNA, indicating a critical role for Ccdc30 in centriole amplification. In Ccdc30-depleted MCCs, basal bodies failed to dock efficiently, accompanied by a significant loss of phalloidin staining, which reflects disruption of the apical actin network. Consequently, the apical surface area of MCCs was significantly reduced (Figure 4L and 4M). Collectively, these findings suggest that, in contrast to Morn2, which functions directly in axoneme assembly, Ccdc30 plays broader roles during MCC development. At earlier stages, Ccdc30 may contribute to MCC fate determination and centriole amplification, during ciliogenesis, it likely supports apical actin organization necessary for basal body migration and docking.(https://doi.org/10.1101/2025.05.20.655211)