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Subcellular localization

cilia, Nucleoplasm, Nucleoli

Functional category

• Signaling (Hedgehog
• GPCRs
• ion channels)

Function

Deficiency of WNT/GSK3 signaling by depletion of cyclin Y and cyclin-Y-like protein 1 induces primary cilia defects in mouse embryonic neuronal precursors, kidney proximal tubules, and adult mice preadipocytes. In serum-starved 293T cells, IF identified highly specific ciliary localization of endogenous CCNY in 95% of cilia analyzed (n = 35) (Figure 4A) and CCNYL1 (Figure 4B), although at a lower frequency (50%, n = 30). CCNY/L1 staining was absent in CCNY/L1DKO cells, confirming specificity of the antibodies (Figures S4A and S4B)(36693320).