CCT3

chaperonin containing TCP1 subunit 3

Tier: Gold Standard Ciliary Genes

Ensembl:
ENSG00000163468
UniProt:
P49368
OMIM:
600114
Synonyms:
CCTG, TRIC5

Ciliogenesis screen results (4 screens)

  • Kim2016: Not Reported
  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-11.23) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:
increased mean platelet volume, decreased mean corpuscular hemoglobin, increased startle reflex, embryonic lethality prior to organogenesis, preweaning lethality, complete penetrance, decreased exploration in new environment

Subcellular localization

basal body, cilia, cytosol

References: PMID:37427378, PMID:40103630, PMID:7961900, PMID:39743588

Functional category

  • Ciliary assembly/disassembly
  • Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
  • Actin & cytoskeleton regulation
  • Metabolism
  • Protein processing & maturation
  • Signaling (Hedgehog, GPCRs, ion channels)
  • Ciliary membrane

Function

USH proteins mutually interact and partially co-localize with the TRiC/CCT subunit CCT3 in the ciliary region of photoreceptor cells and ADGRV1 interacts with the CCT3 subunit of the TriC/CCT chaperonin complex (37427378). Identified by cryo-EM as a component of the radial spoke-associated barrel in sperm flagella (Leung et al., 2025, Nature, PMID 39743588).