CDC20

Cilia effects upon perturbation of CDC20

Cilia number / % ciliated:

No effect

Loss-of-function effect:

Longer cilia

Overexpression effect:

Shorter cilia

Ciliogenesis screen results (4 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-7.81) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

embryonic lethality prior to organogenesis, decreased circulating glucose level, preweaning lethality, complete penetrance, increased circulating glucose level, increased fasting circulating glucose level, increased grip strength, embryonic lethality prior to tooth bud stage, improved glucose tolerance

Mouse ciliopathy phenotype:

increased heart weight

Subcellular localization

basal body, centrosome, cytosol, nucleus

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Actin & cytoskeleton regulation
• Protein processing & maturation

Function

Neuro l differentiation, ciliary formation or D damage repair. Studies suggest that is involved in neuro l connectivity and plasticity (25850036). Potential activity in quiescent and differentiated cells, is recruited to the basal body of the primary cilium, where in complex with APC it negatively regulates ciliary length, by destabilizing axonemal. Inhibition of APC-Cdc20 activity increases the ciliary length, while overexpression of Cdc20 suppresses cilium formation (25139956).

Model organism evidence