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Phenotypes

Mouse phenotype:

increased circulating aspartate transaminase level

Subcellular localization

cilia, Plasma membrane, Actin filaments

Functional category

• Metabolism

Function

Borg3 exhibited the strongest signal in the ciliary compartment compared to Borg1 and Borg2 (Figures 2A and 2B). Similar to septins, structured illumination microscopy (SIM) revealed that Borg proteins primarily localize along the cilium length in a spot-like pattern. While septins exhibited a more even distribution along the cilium, Borg proteins appeared to form more distinct clusters (Figures 2C and 2D). Frequently, Borg3 clusters were distributed along the cilium in an almost equidistant scattering (Video S2). Given the specific localization of Borg proteins at cilia, we analyzed the effect of Borg depletion on cilia formation. IMCD cells were transfected with siRNAs targeting Borg1-3, and after 48 h, siRNA treatment led to a reduction in Borg expression at both the mRNA (Figure S2H) and protein level (Figures 2E–2G). Notably, Borg3 KD significantly reduced the number of cells developing a cilium (Figure 2H and 2I), whereas no such effect was observed for Borg1 or Borg2. Overall, no major changes in cell morphology were observed across all Borg family KDs(40502696).