CDK6

Cilia effects upon perturbation of CDK6

Loss-of-function effect:

No effect

Ciliogenesis screen results (5 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-3.19) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
• Kim et al. 2010 (siRNA) [siRNA]: Longer Cilia (Area per Cilia z=4.28) PMID:20393562

Phenotypes

Mouse phenotype:

increased mean corpuscular volume, abnormal eye morphology, increased mean corpuscular hemoglobin, small superior vagus ganglion

Subcellular localization

basal body, cilia associated gene, cytosol, nucleus

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• T cell biology
• Cell migration & adhesion
• Transcription regulation
• Cilia length regulation

Function

CDK6, together with its cyclin partner cyclin D3, enrich at the ciliary base in RPE-1 cells and an uncanonical CDK6 activity inhibits cilia function by suppressing axoneme polyglutamylation(PMID: 39636239). Consistent with this, knockdown of CDK4/6 with siRNA or CDK4/6 inhibition with Abe also increased cilia length (onsistent with this, knockdown of CDK4/6 with siRNA or CDK4/6 inhibition with Abe also increased cilia length (33127671). CDK6 localizes to cilia base; non-canonical kinase activity promotes cilia disassembly by phosphorylating FIP5-S641, suppressing axoneme polyglutamylation. CDK4/6 inhibitor Abemaciclib restores cilia function in Joubert syndrome cells.