CDKN1B

Cilia effects upon perturbation of CDKN1B

Ciliogenesis screen results (5 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.34) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
• Pusapati et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, neg_rank=523, lfc=-0.71) PMID:30270045

Phenotypes

Mouse phenotype:

decreased bone mineral density, abnormal optic disk morphology, increased spleen weight, decreased mean corpuscular volume, decreased mean corpuscular hemoglobin, increased fasting circulating glucose level, decreased monocyte cell number, decreased circulating triglyceride level, increased circulating alkaline phosphatase level, abnormal startle reflex, decreased bone mineral content, decreased exploration in new environment

Mouse ciliopathy phenotype:

abnormal reti morphology, abnormal reti vasculature morphology

Subcellular localization

basal body, cilia, cytosol, nucleus, transition zone

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Actin & cytoskeleton regulation
• Protein processing & maturation
• Signaling (Hedgehog, GPCRs, ion channels)
• Cardiac & muscle development
• Transition zone
• Transcription regulation

Function

Also known as p27Kip1. Multifunctio l protein that plays crucial roles in cell architecture and migration by regulating rearrangements of the actin cytoskeleton and . p27Kip1 abrogation impairs one of the earliest events of ciliogenesis, docking of the Ehd1- associated preciliary vesicles to the distal appendages of the basal body (32423824).

Model organism evidence