CIT

Cilia effects upon perturbation of CIT

Ciliogenesis screen results (4 screens)

• Kim2016: No effect
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.96) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

increased circulating amylase level, preweaning lethality, complete penetrance

Subcellular localization

cilia associated gene

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Actin & cytoskeleton regulation
• Transcription regulation

Function

CK2α interacts with CIT-K, localizes at the midbody through CIT-K and affects cytokinesis through TUBB3 S444 phosphorylation. Asynchronous HeLa cells were processed for immunofluorescence using the anti-CK2α and anti-Citron antibodies. The two proteins colocalize at the midbody of late telophase cells. Asynchronous HeLa cells were processed for immunofluorescence 48 h after transfection with the indicated siR s using the anti-CK2α and anti-α-tubulin (α-TUB). The region containing the midbody of two dividing cells was magnified in the inset( 26586574).