CNGA1

cyclic nucleotide gated channel subunit alpha 1

Ensembl:
ENSG00000198515
UniProt:
P29973
OMIM:
123825
Synonyms:
CNCG, CNCG1, CNG1, RCNC1, RCNCA

Cilia effects upon perturbation of CNGA1

Ciliogenesis screen results (4 screens)

  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-6.84) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
  • Elliott et al. 2025 (CRISPRa) [CRISPRa]: Disassembly Trigger (casTLE Effect=-3.62) PMID:41160700

Phenotypes

Mouse phenotype:
decreased total reti thickness, abnormal reti outer nuclear layer morphology
Human ciliopathy phenotype:
retinitis pigmentosa; retinitis pigmentosa 49

Subcellular localization

cilia

Functional category

  • Ciliary assembly/disassembly
  • Signaling (Hedgehog, GPCRs, ion channels)
  • Ciliary membrane

Function

Subunit of the cyclic nucleotide-gated (Cng) channel localised to the outer segment. Mutations in Cnga1 which have been associated with retinitis pigmentosa in humans (25319694)

Model organism evidence

C. elegans (3 references)

Notably, deactivating the cyclic nucleotide-gated (CNG) channel TAX-4 on the ciliary membrane promotes lifespan extension through a UPRER-dependent mechanism.

We demonstrate that ciliary localization of CNG channel subunits is interdependent on different channel subunits in specific cells, and identify sequences required for efficient ciliary targeting and localization of the TAX-2 CNGB and TAX-4 CNGA subunits.

PMIDs: 38857399, 23886944, 21124868

Xenopus (1 reference)

Consistent with retrograde trafficking, the axoneme, which potentially mediates retrograde intraflagellar trafficking, runs through the entire axis of OSs.

PMIDs: 24855015

Mus musculus (1 reference)

The daf-25 dauer phenotype is suppressed by daf-10/IFT122 mutations (which disrupt ciliogenesis), but not by daf-6/PTCHD3 mutations (which prevent environmental exposure of sensory cilia), implying that DAF-25 functions in the cilia themselves.

PMIDs: 21124868