?

Subcellular localization

Ciliary associated gene, Nucleoplasm, Centrosome, Cytosol

Functional category

• Metabolism
• Non-motile cilium / primary cilium
• Trafficking (BBSome
• small GTPases
• vesicular transport
• ATPases)
• Ciliary assembly/disassembly

Function

We show that CENTLEIN depletion causes the persistent accumulation of RABIN8 on the pericentrosome and primary cilium loss in hTERT-immortalized retinal pigment epithelial cells and murine embryonic fibroblasts. CENTLEIN interacts with RABIN8 directly. A stretch of a 31-amino acid sequence located in the 200‒230 region of the RABIN8 GEF domain is responsible for its physical interaction with CENTLEIN, while expression of the full-length but not the internal deletion lacking the RABIN8-binding site of CENTLEIN largely rescues the ciliogenesis defect provoked by CENTLEIN depletion. Expression of activated RAB8A partially reverses cilium loss in CENTLEIN-null RPE1 cells, so the functional importance of the CENTLEIN-RABIN8 interaction is defined(37475549).