DAAM1

dishevelled associated activator of morphogenesis 1

Ensembl:
ENSG00000100592
UniProt:
Q9Y4D1
OMIM:
606626
Synonyms:
KIAA0666

Cilia effects upon perturbation of DAAM1

Ciliogenesis screen results (3 screens)

  • Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:
increased blood urea nitrogen level, preweaning lethality, complete penetrance

Subcellular localization

cilia, cytosol

Functional category

  • Ciliary assembly/disassembly
  • Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
  • Actin & cytoskeleton regulation

Function

DAAM1 is present within the the actin-rich base of multiciliated epidermal cells of the X. laevis skin where it regulates the actin network that stabilizes cilia. Loss of Daam1 in motile cilia results in their loss of polarity(26644512). Loss of the PCP component Daam1 negatively regulates ciliogenesis in MDCKII and IMCD3 cells. Daam1 knockdown within these mammalian kidney epithelial cells result in the formation of fewer cilia. Ciliogenesis in MDCKII cells depends on the formin activity of Daam1. In MDCKII and IMCD3 cells Daam1 localizes to vesicles that carry ciliary components(31469868).

Model organism evidence

Danio rerio (1 reference)

Divergent roles of the Wnt/PCP Formin Daam1 in renal ciliogenesis.

PMIDs: 31469868

Xenopus (2 references)

Divergent roles of the Wnt/PCP Formin Daam1 in renal ciliogenesis.

Developmentally regulated GTP-binding protein 1 modulates ciliogenesis via an interaction with Dishevelled.

PMIDs: 31469868, 31270137