DCP2

Cilia effects upon perturbation of DCP2

Cilia number / % ciliated:

No effect

Loss-of-function effect:

No effect

Ciliogenesis screen results (4 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, casTLE effect=-1.53) PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
• Pusapati et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, neg_rank=749, lfc=-2.20) PMID:30270045

Phenotypes

Mouse phenotype:

abnormal coat/hair pigmentation; increased heart weight; preweaning lethality; complete penetrance

Subcellular localization

cell junctions, cilia associated gene, cytoplasmic bodies, nucleoplasm

Functional category

• Ciliary morphology regulation
• mRNA metabolism

Function

DCAP-1 and DCAP-2, which are the homologues of mammalian DCP1 and DCP2 mRNA decapping enzymes, respectively, are involved in formation of dual rod-type and wing-like shaped cilia in C. elegans. mRNA decapping enzyme catalyzeshydrolysis of 5′ cap structure of mRNA, which leads to degradation of mRNA. Rescue experiments showed that DCAP-2 acts not in glial cells surrounding cilia but in neurons. This is the first evidence to demonstrate that mRNA decapping is involved in ciliary shape formation(PMID: 28887031).

Model organism evidence