DENND1A

Cilia effects upon perturbation of DENND1A

Cilia number / % ciliated:

Unknown

Loss-of-function effect:

No effect

Overexpression effect:

Unknown

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Elliott et al. 2025 (CRISPRa) [CRISPRa]: Disassembly Trigger (casTLE Effect=-2.26) PMID:41160700

Phenotypes

Mouse phenotype:

abnormal neural tube morphology, abnormal hindbrain development, pretal lethality prior to heart atrial septation, preweaning lethality, complete penetrance, abnormal forebrain development, embryonic growth retardation, abnormal placenta morphology

Mouse ciliopathy phenotype:

abnormal neural tube closure, abnormal embryo turning, abnormal heart morphology

Subcellular localization

cilia associated gene

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)

Function

DENND1A is a guanine nucleotide exchange factor (GEF) that activates Rab GTPases, including Rab35. It plays a role in membrane trafficking processes, which are essential for ciliary function(PMID: 31432619).