DENND1A

Cilia effects upon perturbation of DENND1A

Cilia number / % ciliated:

Unknown

Loss-of-function effect:

No effect

Overexpression effect:

Unknown

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Elliott et al. 2025 (CRISPRa) [CRISPRa]: Disassembly Trigger (casTLE Effect=-2.26) PMID:41160700

Phenotypes

Mouse phenotype:

abnormal neural tube morphology, abnormal hindbrain development, pretal lethality prior to heart atrial septation, preweaning lethality, complete penetrance, abnormal forebrain development, embryonic growth retardation, abnormal placenta morphology

Subcellular localization

No ciliary localization reported (cilia-associated gene).

References: PMID:31432619

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)

Function

DENND1A is a guanine nucleotide exchange factor (GEF) that activates Rab GTPases, including Rab35. It plays a role in membrane trafficking processes, which are essential for ciliary function(PMID: 31432619).