DLG1

Cilia effects upon perturbation of DLG1

Cilia number / % ciliated:

No effect

Loss-of-function effect:

Longer cilia

Overexpression effect:

no effect

Ciliogenesis screen results (4 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.37) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Subcellular localization

cilium, basal body

References: PMID:37987012, PMID:42358734, PMID:41005307, PMID:38849673

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Actin & cytoskeleton regulation
• Cell migration & adhesion
• Signaling (Hedgehog, GPCRs, ion channels)
• Cardiac & muscle development
• Muscle contraction & physiology
• Cilia length regulation

Function

DLG1, a core component of the Scribble polarity complex, controls ciliary protein composition and cilium length in mouse kidney epithelial cells (PMID 37987012). Proximity-dependent biotinylation in ciliated IMCD3 cells identified 46 high-confidence DLG1 interactors, 35 of them previously linked to ciliary or centrosomal networks; ZDHHC5 was confirmed as an interactor at primary cilia, and DLG1 is required for correct STXBP1 localization at the ciliary base. Depletion of zdhhc5a/zdhhc5b in zebrafish produces elongated cilia and ciliopathy-like phenotypes (PMID 42358734).