DRG1

developmentally regulated GTP binding protein 1

Tier: Gold Standard Ciliary Genes

Ensembl:
ENSG00000185721
UniProt:
Q9Y295
OMIM:
603952
Synonyms:
NEDD3

Cilia effects upon perturbation of DRG1

Loss-of-function effect:
Shorter cilia

Ciliogenesis screen results (5 screens)

  • Kim2016: Not Reported
  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-10.94) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, casTLE effect=-1.01) PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
  • Pusapati et al. 2018 (CRISPR) [CRISPR]: Negative Regulator (Hh signaling, pos_rank=51, lfc=0.88) PMID:30270045

Phenotypes

Mouse phenotype:
preweaning lethality, incomplete penetrance, abnormal skin morphology, increased red blood cell distribution width, abnormal salivary gland morphology

Subcellular localization

basal body

References: PMID:31270137

Functional category

  • Ciliary assembly/disassembly
  • Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
  • Actin & cytoskeleton regulation

Function

Drg1 as a novel binding partner of Dvl that is required for the proper Dvl interaction with Daam1. These interactions play a crucial role in modulating the formation of the apical actin meshwork and basal body docking in the MCCs(31270137). Drg1 knockdown decreases the length of cilia.