DRG1

developmentally regulated GTP binding protein 1

Ensembl:
ENSG00000185721
UniProt:
Q9Y295
OMIM:
603952
Synonyms:
NEDD3

Cilia effects upon perturbation of DRG1

Loss-of-function effect:
Shorter cilia

Ciliogenesis screen results (5 screens)

  • Kim2016: Not Reported
  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-10.94) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, casTLE effect=-1.01) PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
  • Pusapati et al. 2018 (CRISPR) [CRISPR]: Negative Regulator (Hh signaling, pos_rank=51, lfc=0.88) PMID:30270045

Phenotypes

Mouse phenotype:
preweaning lethality, incomplete penetrance, abnormal skin morphology, increased red blood cell distribution width, abnormal salivary gland morphology

Subcellular localization

basal body

Functional category

  • Ciliary assembly/disassembly
  • Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
  • Actin & cytoskeleton regulation

Function

Drg1 as a novel binding partner of Dvl that is required for the proper Dvl interaction with Daam1. These interactions play a crucial role in modulating the formation of the apical actin meshwork and basal body docking in the MCCs(31270137). Drg1 knockdown decreases the length of cilia.

Model organism evidence

Xenopus (1 reference)

Using the Xenopus laevis embryo, we show that Dishevelled (Dvl), a key Wnt signaling scaffold that is critical to proper ciliogenesis, interacts with Drg1 (developmentally regulated GTP-binding protein 1).

PMIDs: 31270137