DYNC2LI1

dynein cytoplasmic 2 light intermediate chain 1

Tier: Gold Standard Ciliary Genes

Ensembl:
ENSG00000138036
UniProt:
Q8TCX1
OMIM:
617083
Synonyms:
CGI-60, D2LIC, DKFZP564A033, LIC3

Cilia effects upon perturbation of DYNC2LI1

Cilia number / % ciliated:
Decreased cilia number
Loss-of-function effect:
Longer cilia
Overexpression effect:
no effect

Ciliogenesis screen results (3 screens)

  • Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, casTLE effect=-5.95) PMID:29459680
  • Elliott et al. 2025 (CRISPRa) [CRISPRa]: Disassembly Trigger (casTLE Effect=-3.53) PMID:41160700

Ciliopathy associations

  • Ellis-van Creveld Syndrome
  • Short-Rib Thoracic Dysplasia
  • Skeletal Ciliopathy

References: PMID:28857138, PMID:26077881, PMID:35927022, PMID:29458881, PMID:33030252, PMID:40396377

Subcellular localization

basal body, cilia, cytosol

References: PMID:41005307, PMID:26077881

Functional category

  • Ciliary assembly/disassembly
  • Actin & cytoskeleton regulation
  • Transition zone
  • Cilia length regulation

Function

Component of the dynein-2 complex. DYNC2LI1 knockdown led to significantly increased variation in cilia length.