EIF4G1

Cilia effects upon perturbation of EIF4G1

Ciliogenesis screen results (5 screens)

• Kim2016: No effect
• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: Hyper-ciliogenesis (robust z=2.30, ciliated=55.2%) PMID:26595381
• Elliott et al. 2025 (CRISPRa) [CRISPRa]: Disassembly Trigger (casTLE Effect=-2.67) PMID:41160700

Phenotypes

Mouse phenotype:

increased fasting circulating glucose level, increased total body fat amount, decreased spleen weight, increased total reti thickness, decreased lean body mass, preweaning lethality, complete penetrance, decreased bone mineral density, abnormal vocalization

Mouse ciliopathy phenotype:

abnormal reti blood vessel morphology, increased circulating aspartate transamise level, increased circulating alanine transamise level, abnormal reti vasculature morphology

Subcellular localization

cilia, cytosol, microtubules, ribosome

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• Viral interactions
• Signaling (Hedgehog, GPCRs, ion channels)

Function

Eif3m and Eif4g were distributed as puncta close to axonemal outer MT doublets . Ependymal multicilia contain ribosomes, eIFs, and R (34848703).