?

Subcellular localization

basal body, Plasma membrane, Microtubules, Cytosol

Functional category

• Motile cilium & axoneme
• Ciliary assembly/disassembly

Function

The SAG-induced ciliary accumulation of endogenous Gli2 is reduced in NIH/3T3 cells with Sec3/5/8 knockdown (Supplementary Fig. S2c). Importantly, exocyst loss of function does not reduce the total number of ciliated cells, suggesting that it does not have a broad effect on ciliogenesis under experimental conditions that we used (Supplementary Fig. S2d). Having demonstrated that the exocyst is required for the trafficking of Gli to cilia, we wondered if the localization of the exocyst is affected by Hh pathway activation. Indeed, the treatment with SAG increases the amount of Sec3 and Sec5 at the ciliary base (Fig. 4e). Similar to Gli2, ciliary accumulation of HA-Lkb1 also dropped after the shRNA knockdown of Sec3/5/8 (Fig. 8c)(38378792).