FASN

Cilia effects upon perturbation of FASN

Cilia number / % ciliated:

Incrased cilia number

Overexpression effect:

Reduced cilia numbers

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-23.11) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, casTLE effect=-0.88) PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Subcellular localization

cilia associated gene

Functional category

• Ciliary assembly/disassembly
• Metabolism
• Signaling (Hedgehog, GPCRs, ion channels)

Function

SREBP1c-induced loss of the primary cilium could be confirmed in mammalian Madin-Darby canine kidney (MDCK) cells and was mediated by changes in the supply of fatty acids. In the second experiment, we transfected MDCK cells with expression constructs encoding FASN and acetyl-CoA carboxylase, which are the two downstream targets of nSREBP1c that catalyze fatty acid synthesis. Expression of FASN- and acetyl-CoA carboxylase significantly reduced the percentage of ciliated cells from 79% for control cells to 27% for FASN- and acetyl-CoA carboxylase–transfected cells (Fig. 4A) (20889723)