G3BP1

Cilia effects upon perturbation of G3BP1

Cilia number / % ciliated:

No effect

Loss-of-function effect:

Longer cilia

Overexpression effect:

Unknown

Ciliogenesis screen results (2 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680

Phenotypes

Mouse phenotype:

abnormal skin coloration, preweaning lethality, incomplete penetrance, decreased circulating alkaline phosphatase level, abnormal coat appearance, decreased total reti thickness, decreased prepulse inhibition

Mouse ciliopathy phenotype:

abnormal reti morphology

Subcellular localization

cilia associated gene

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Actin & cytoskeleton regulation
• Metabolism
• Viral interactions

Function

G3BP1 and G3BP2 do not affect cilium formation but regulate cilium length. In addition, the extent of cilium elongation in G3BP dKO cells was greater than that in G3BP1 KO or G3BP2 KO cells , suggesting that both G3BP1 and G3BP2 may regulate cilium elongation in parallel(38088586).