GOLGB1

Cilia effects upon perturbation of GOLGB1

Cilia number / % ciliated:

Decreased cilia number (in depletion)

Loss-of-function effect:

Longer (partial depletion) Failure of ciliogenesis (severe depletion)

Ciliogenesis screen results (4 screens)

• Kim2016: Not Reported
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
• Kim et al. 2010 (siRNA) [siRNA]: Longer Cilia (Area per Cilia z=4.52) PMID:20393562

Subcellular localization

cilia

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• Signaling (Hedgehog, GPCRs, ion channels)
• Cilia length regulation

Function

Required for ciliogenesis and control of ciliary length, through the lregulation of pericentrosomal location of the dynein-2 intermediate chain WDR34. Depletion results in cilia number and structure (fewer but longer cilia) (28546340, 24046448). Partial depletion of giantin or of WDR34 leads to an increase in cilia length consistent with the concept that giantin acts through dynein-2. Suppression of giantin … leads to inability of cells to form primary cilia. (24046448) Knockdown in zebrafish … cilia number was reduced and visible cilia were longer. (23391289)

Model organism evidence