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Subcellular localization

cilia

Functional category

• Motile cilium & axoneme
• Signaling (Hedgehog
• GPCRs
• ion channels)

Function

We found that the OCN receptor GPR158 is present at the PC of hippocampal neurons and mediates the regulation of autophagy machinery by OCN. Remarkably, we identified five ciliary-targeting VxPx motifs at the C-terminal domain of GPR158, suggesting its possible localization in the PC (Extended Data Fig. 1f). Co-staining of endogenous GPR158 with neuronal ciliary protein adenylate cyclase 3 (ACIII) indicates its presence, at least partially, at the PC in hippocampal neurons, in cultures (in approximatively 75% of primary hippocampal neurons) and in mouse hippocampi (Fig. 1c and Extended Data Fig. 1g–j). Furthermore, we found that OCN treatment increases the number of GPR158-positive puncta associated with ACIII-positive ciliary plasma membrane (Fig. 1c)(39984747).