GPR22

Cilia effects upon perturbation of GPR22

Loss-of-function effect:

Abnormal / altered cilia

Overexpression effect:

Abnormal / altered

Ciliogenesis screen results (4 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-5.97) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

abnormal gait, decreased total body fat amount, decreased erythrocyte cell number, decreased hemoglobin content, hyperactivity, increased lean body mass, decreased hematocrit, increased circulating potassium level, decreased circulating serum albumin level, increased circulating phosphate level

Mouse ciliopathy phenotype:

increased circulating aspartate transamise level, increased circulating alanine transamise level, increased circulating creatinine level

Subcellular localization

cilia

Functional category

• Ciliary assembly/disassembly
• Signaling (Hedgehog, GPCRs, ion channels)
• Cilia length regulation

Function

Regulation of Left-Right asymmetry through the Kupffer鈥檚 vesicle (KV) and mantains cilia length and structure in KV. Both knockdown and overexpression of gpr22 in the Kupffer’s vesicle result in abnormal cilia length and ultrastructure, leading to defects in left–right axis formation. (25335082)

Model organism evidence