GSK3B

Cilia effects upon perturbation of GSK3B

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Ciliogenesis blocked

Ciliogenesis screen results (6 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: Negative Regulator (Hh signaling, casTLE effect=0.61) PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
• Pusapati et al. 2018 (CRISPR) [CRISPR]: Negative Regulator (Hh signaling, pos_rank=283, lfc=0.74) PMID:30270045
• Failler/Valderrama et al. 2021 (siRNA) [siRNA]: Negative Regulator (Ciliogenesis Candidate) PMID:33206585

Subcellular localization

basal body, centrosome, cytosol, microtubules, nucleus

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Actin & cytoskeleton regulation
• Viral interactions
• Cell migration & adhesion
• Signaling (Hedgehog, GPCRs, ion channels)
• Transcription regulation
• Cilia length regulation

Function

Involved in primary cilia elongation, microtubule assembly, stability and dy mics. Modulates CRMP- 2 ciliary localisation, and its ephosphorylated state is required for ciliary function (23185275). Ciliary- mainte nce sig lling network, its disruption causes loss of cilia (17450132). Phosphorylates Sufu and stabilises it against Shh sig ling-induced degradation (21317289). Ciliogenesis was inhibited … by knockout of GSK3β in cells. Gsk3b modulates cilia length through Torc1 signaling. GSK3 inhibition by lithium induces long cilia phenotype. Combined inactivation of pVHL and GSK3β leads to loss of cilia. (17450132) (22308353)