HIF1A

hypoxia inducible factor 1 subunit alpha

Tier: Cilia-Associated Genes

Ensembl:
ENSG00000100644
UniProt:
Q16665
OMIM:
603348
Synonyms:
BHLHE78, HIF-1ALPHA, HIF1, MOP1, PASD8

Cilia effects upon perturbation of HIF1A

Loss-of-function effect:
Prevents cilia loss

Ciliogenesis screen results (6 screens)

  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-4.39) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
  • Pusapati et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, neg_rank=432, lfc=-1.73) PMID:30270045
  • Failler/Valderrama et al. 2021 (siRNA) [siRNA]: Negative Regulator (Ciliogenesis Candidate) PMID:33206585
  • Elliott et al. 2025 (CRISPRa) [CRISPRa]: Disassembly Trigger (casTLE Effect=-3.50) PMID:41160700

Subcellular localization

cytosol, nucleus

References: PMID:31357086, PMID:24378640, PMID:22084569, PMID:39714449

Functional category

  • Ciliary assembly/disassembly
  • Actin & cytoskeleton regulation
  • Metabolism
  • Neurogenesis & migration
  • Protein processing & maturation
  • Cell migration & adhesion
  • Signaling (Hedgehog, GPCRs, ion channels)
  • Cardiac & muscle development
  • ECM & connective tissue
  • Transcription regulation

Function

Maintains the chondrocytes healthy under hypoxia ( 31357086). Stabilised by USP8 through deubiquitination, which is essential for its ciliogenesis- promoting function ( 24378640). Gene silencing of HIF-1α prevented cilia loss under hypoxia, suggesting hypoxia impacts ciliogenesis via a HIF-1α / Wnt signaling axis. (22084569) One possible explanation for the reduced ciliogenesis or increased ciliary disassembly is the functional relationship between HIF and pVHL. (39714449) USP8 deubiquitination of HIF1α is essential to maintain ciliogenesis. (24378640)