HSPA5

Cilia effects upon perturbation of HSPA5

Loss-of-function effect:

Shorter cilia

Ciliogenesis screen results (4 screens)

• Kim2016: No effect
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-5.09) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: Hyper-ciliogenesis (robust z=2.37, ciliated=55.8%) PMID:26595381

Phenotypes

Mouse phenotype:

preweaning lethality, complete penetrance, increased circulating hdl cholesterol level, increased circulating cholesterol level, embryonic lethality prior to tooth bud stage, embryonic lethality prior to organogenesis, decreased circulating chloride level

Subcellular localization

flagella, microtubules, nucleus

References: PMID:28710093, PMID:40245563

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• Metabolism
• Protein processing & maturation
• Signaling (Hedgehog, GPCRs, ion channels)

Function

HSPA5 localized to flagella (25613900) The most abundant membrane-associated proteins were heat-shock protein family A (Hsp70) member (Hspa5) … (28710093) Hspa5 was significantly downregulated following lithium carbonate exposure, and decreased Cdc42 expression and cytoskeletal abnormalities could disrupt normal cilia development and lead to shortened cilia. (40245563)