IDE

Cilia effects upon perturbation of IDE

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Shorter cilia

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-6.43) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

increased circulating amylase level, decreased total body fat amount, process of degenerative change, abnormal incisor morphology, developmental and structural abnormality, abnormal maxilla morphology, increased lean body mass, abnormal hair texture

Mouse ciliopathy phenotype:

abnormal cranium morphology, increased heart weight, cyst

Subcellular localization

cilia associated gene, cytosol, mitochondria, nucleus, peroxisome

Functional category

• Ciliary assembly/disassembly
• T cell biology
• Viral interactions
• Signaling (Hedgehog, GPCRs, ion channels)

Function

IDE deficiency triggers impairments in cilia formation, inducing alpha cell hyperplasia and possibly also contributing to dysregulated glucagon secretion and hyperglucago emia ( 35652923). IDE deficiency triggers impairments in cilia formation, inducing alpha cell hyperplasia (35652923) We found a decreased number of cilia in siRNA-Ide cells compared to its control. In addition, we observed that the average length of primary cilia was significantly shorter in siRNA-Ide αTC1.9 cells compared to control cells, indicating that the knockdown of Ide affects correct ciliogenesis.