IFT88

Cilia effects upon perturbation of IFT88

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Shorter cilia

Overexpression effect:

No effect

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-6.16) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, casTLE effect=-4.99) PMID:29459680
• Pusapati et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, neg_rank=650, lfc=-3.11) PMID:30270045

Phenotypes

Mouse phenotype:

embryonic growth retardation, preweaning lethality, complete penetrance, abnormal embryo size

Subcellular localization

basal body, centrosome, cilia

References: PMID:40076734, PMID:41005307, PMID:11062270

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• Reproduction & sperm
• Signaling (Hedgehog, GPCRs, ion channels)

Function

Component of IFT complex B. Required for ciliary and flagellar assembly and may play a role in the attachment of the IFT particle to its cargo or to the anterograde IFT motor FLA10-kinesin-II (11062270).