KATNB1

Cilia effects upon perturbation of KATNB1

Loss-of-function effect:

Decreased cilia number

Ciliogenesis screen results (4 screens)

• Kim2016: Not Reported
• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, casTLE effect=-1.05) PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

preweaning lethality, complete penetrance

Subcellular localization

centrosome, flagella, nucleus

References: PMID:27717557, PMID:25521379, PMID:25521378, PMID:34822718

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• Reproduction & sperm

Function

katanin p80 is not only involved in the formation of manchette and sperm head shape, but also in the correct formation of the flagellum as katanin p80 is also localized in the distal region of step III round spermatids (27717557). KATNB1 regulates overall centriole, mother centriole, and cilia number. (25521379) Katnb1 knockout in mice leads to … affecting centriole and cilia biogenesis during development. (25521378) KATNB1 is the p80 regulatory subunit that directs katanin activity and is necessary for normal cilia formation. ( 34822718)