KCNF1

potassium voltage-gated channel modifier subfamily F member 1

Ensembl:
ENSG00000162975
UniProt:
Q9H3M0
OMIM:
603787
Synonyms:
IK8, KCNF, KH1, KV5.1

Cilia effects upon perturbation of KCNF1

Loss-of-function effect:
Knocdown impairs ciliogenesis

Ciliogenesis screen results (4 screens)

  • Kim2016: Not Reported
  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-4.54) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:
hyperactivity, decreased locomotor activity, abnormal behavior, decreased anxiety-related response, decreased thigmotaxis

Subcellular localization

cilia, transition zone

Functional category

  • Ciliary assembly/disassembly
  • Signaling (Hedgehog, GPCRs, ion channels)
  • Transition zone

Function

Ion channel gene that exhibits a significant negative effect on ciliogenesis but not on cell number. Potential new candidate in human ciliopathies (26546361). To validate the effect of knockdowns of … Kcnf1 … on ciliogenesis we repeated the siRNA knockdowns in mIMCD3 cells. (26546361) KCNF1 localizes to primary cilia and regulates ciliogenesis. (26546361)

Model organism evidence

Mus musculus (1 reference)

Screen-based identification and validation of four new ion channels as regulators of renal ciliogenesis.

PMIDs: 26546361