KIF27

Cilia effects upon perturbation of KIF27

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Shorter cilia

Overexpression effect:

Unknown

Ciliogenesis screen results (4 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.94) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Basu et al. 2023 (siRNA) [siRNA]: Left-Right Asymmetry Candidate (Z2MCilia=-1.03) PMID:37771269
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Ciliopathy associations

• Acrocallosal Syndrome

Subcellular localization

basal body

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• Metabolism
• Cilia length regulation

Function

Essential for pla rian ciliogenesis (19933103). Kif27 expression is upregulated during mouse tracheal epithelial cells differentiation, consistent with their essential roles in motile ciliogenesis (19305393). Interacts with Fu and both may regulate central pair assembly, probably through the recruitment or activation of central pair proteins such as Spag16 and Pcdp1. It could also interact with basal body components or accessory structures in controlling cilia orientation (23907739).Using scanning electron microscopy (SEM), reduced ciliation in the brain ependyma and morphological defects for respiratory motile cilia including reduced cilia length observed , supporting a role for KIF27 in motile cilia biogenesis.