KIF3B

kinesin family member 3B

Ensembl:
ENSG00000101350
UniProt:
O15066
OMIM:
603754
Synonyms:
FLA8, KIAA0359, KLP-11

Cilia effects upon perturbation of KIF3B

Cilia number / % ciliated:
Unknown
Loss-of-function effect:
Shorter cilia
Overexpression effect:
Unknown

Ciliogenesis screen results (4 screens)

  • Kim2016: No effect
  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.13) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, casTLE effect=-4.37) PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:
preweaning lethality, complete penetrance, increased bone mineral content, pretal lethality prior to heart atrial septation, increased grip strength
Human ciliopathy phenotype:
retinitis pigmentosa 89

Ciliopathy associations

  • Biliary Atresia
  • Retinal Dystrophy/Degeneration

Subcellular localization

centrosome, cilia, cytosol, endosome

Functional category

  • Ciliary assembly/disassembly
  • Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
  • Actin & cytoskeleton regulation
  • Metabolism
  • T cell biology
  • Signaling (Hedgehog, GPCRs, ion channels)

Function

Essential for ciliogenesis and the left鈥搑ight asymmetry through intraciliary transportation of materials for ciliogenesis of motile cilia that produces the nodal flow (9865700). Its phosphorylation inhibits IFT entry and flagellar lengthening restricting flagellar assembly (30057303). The cilia in the KIF3B’-YFP/FLA10/KAP cells had an average length of 10.6 ± 1.1 μm (n = 50),~15% shorter compared to the control cells (12.6 ± 1.3 μm, n = 50) and WT cells (PMID: 33112235).

Model organism evidence

Mus musculus (2 references)

While the kinesin-2 motors KIF3A and KIF3B have essential roles in ciliogenesis and Hedgehog (HH) signal transduction, potential role(s) for another kinesin-2 motor, KIF17, in HH signaling have yet to be explored.

Heterodimeric kinesin-2 (KIF3A/KIF3B/KAP3) powers several intracellular transport processes, including intraflagellar transport (IFT), essential for ciliogenesis.

PMIDs: 38669326, 38405607