KIF3B

Cilia effects upon perturbation of KIF3B

Cilia number / % ciliated:

Unknown

Loss-of-function effect:

Shorter cilia

Overexpression effect:

Unknown

Ciliogenesis screen results (4 screens)

• Kim2016: No effect
• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.13) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, casTLE effect=-4.37) PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

preweaning lethality, complete penetrance, increased bone mineral content, pretal lethality prior to heart atrial septation, increased grip strength

Human ciliopathy phenotype:

retinitis pigmentosa 89

Ciliopathy associations

• Biliary Atresia
• Retinal Dystrophy/Degeneration

References: PMID:32386558, PMID:41405813

Subcellular localization

centrosome, cilia, cytosol, endosome, ciliary tip

References: PMID:41005307, PMID:33112235

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Actin & cytoskeleton regulation
• Metabolism
• Signaling (Hedgehog, GPCRs, ion channels)

Function

Essential for ciliogenesis and the left–right asymmetry through intraciliary transportation of materials for ciliogenesis of motile cilia that produces the nodal flow (9865700). Its phosphorylation inhibits IFT entry and flagellar lengthening restricting flagellar assembly (30057303). The cilia in the KIF3B’-YFP/FLA10/KAP cells had an average length of 10.6 ± 1.1 μm (n = 50),~15% shorter compared to the control cells (12.6 ± 1.3 μm, n = 50) and WT cells (PMID: 33112235).