KIF9

Cilia effects upon perturbation of KIF9

Cilia number / % ciliated:

No effect

Loss-of-function effect:

Shorter cilia

Overexpression effect:

Unknown

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

small spleen, small liver, developmental dysplasia, abnormal skin morphology

Subcellular localization

basal body, cilia, ciliary tip

References: PMID:41005307, PMID:35531639

Functional category

• Ciliary assembly/disassembly
• Actin & cytoskeleton regulation
• Metabolism
• Reproduction & sperm
• Cell migration & adhesion

Function

Kif9 is necessary for ciliary motility and the proper distal localization of not only central pair proteins, but also radial spokes and dynein arms (35531639). Kif9 is integral for ciliary beating and is necessary for proper axonemal distal end integrity.(PMID: 35531639) KIF9 loss causes centriolar satellite aggregation near centrosome, leading to cilia length defects. Alters levels of TALPID3, CEP131, CEP170, and CEP290. Disrupts cilia length without necessarily affecting ciliation frequency.