LARP6

Cilia effects upon perturbation of LARP6

Cilia number / % ciliated:

Decreased cilia number

Ciliogenesis screen results (2 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Hyper-ciliogenesis (z=2.55) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680

Phenotypes

Mouse phenotype:

increased circulating alkaline phosphatase level, increased circulating triglyceride level, abnormal eye morphology, abnormal skin morphology, abnormal semil vesicle morphology

Subcellular localization

nucleus

References: PMID:28344782

Functional category

• Ciliary assembly/disassembly

Function

The binding of LARP6 and DNAAF6 in dynein axonemal particles regulates highly expressed α-tubulin protein near the apical side of Xenopus MCCs during ciliogenesis. Knockdown of LARP6 in Xenopus embryos resulted in neural tube closure defects that were coincident with the absence of cilia. The absence of cilia at the epidermis was also observed in LARP6 morphants, and this defect was due to the absence of basal bodies at the apical cell surface. (28344782)