LIMK2

Cilia effects upon perturbation of LIMK2

Cilia number / % ciliated:

Incrased cilia number

Ciliogenesis screen results (5 screens)

• Kim2016: No effect
• Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: Hyper-ciliogenesis (robust z=3.10, ciliated=61.3%) PMID:26595381
• Kim et al. 2010 (siRNA) [siRNA]: Longer Cilia (Area per Cilia z=5.40) PMID:20393562

Phenotypes

Mouse phenotype:

preweaning lethality, incomplete penetrance, increased startle reflex, abnormal locomotor activation, increased mean corpuscular hemoglobin concentration, long tibia, increased body length

Subcellular localization

basal body, nucleus

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Actin & cytoskeleton regulation

Function

Actin remodelling factor. Role in the negative regulation of ciliogenesis by upregulating the expression of YAP/TAZ target genes. Together with TESK1, LIMK2 plays a role in suppressing the directio l trafficking of ciliary vesicles to the ciliary base (25849865). knockdown of LIMK2 significantly increased the number of ciliated cells. (25849865)