MAP9

microtubule associated protein 9

Ensembl:
ENSG00000164114
UniProt:
Q49MG5
OMIM:
610070
Synonyms:
ASAP, FLJ21159

Cilia effects upon perturbation of MAP9

Loss-of-function effect:
Shorter cilia
Overexpression effect:
Longer cilia

Ciliogenesis screen results (2 screens)

  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-2.12) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680

Subcellular localization

basal body, cilia

Functional category

  • Ciliary assembly/disassembly
  • Actin & cytoskeleton regulation
  • Signaling (Hedgehog, GPCRs, ion channels)

Function

MAP9, origi lly identified as a MT-binding protein stabilizing MT structure during both mitosis and interphase in human cell lines (16049101). MAP9 has a role in the formation and function of primary cilia, specific expression of the C. elegans MAP9 ortholog MAPH-9 in ciliated sensory neurons (27930654, 33798428), map9 depletion induced developmental abnormalities related to cilia-dependent sig ling pathways in zebrafish (24553125), and MAP9 localized in the photoreceptor CC region of canine reti (28993665). MAP9 is prominently localized in the basal body of primary cilia and played an important role in maintaining the structure of ciliary microtubule axoneme(37650070).

Model organism evidence

Mus musculus (1 reference)

Loss of MAPH-9 caused ultrastructural MTD defects, dysregulated axonemal motor velocity, and perturbed cilia function.

PMIDs: 36865107

C. elegans (2 references)

Loss of MAPH-9 caused ultrastructural MTD defects, dysregulated axonemal motor velocity, and perturbed cilia function.

Whole-Organism Developmental Expression Profiling Identifies RAB-28 as a Novel Ciliary GTPase Associated with the BBSome and Intraflagellar Transport.

PMIDs: 36865107, 27930654

Danio rerio (1 reference)

Microtubules are structural components of the cell cytoskeleton and key factors for mitosis and ciliogenesis in eukaryotes.

PMIDs: 24553125