?

Subcellular localization

basal body, Nucleoplasm, Cytosol

Functional category

• Metabolism
• Ciliary assembly/disassembly
• Cilia–cytoskeleton/adhesion links

Function

We identified another A-C linker component, MIIP (migration and invasion inhibitory protein). Using U-ExM in combination with cell biology approaches, we revealed two crucial functions of the A-C linker proteins, CCDC77, WDR67, and MIIP, in centriole structural integrity but also in duplication through torus assembly. Collectively, this study establishes the role of the A-C linker in centriole function, offering a foundation for exploring its broader significance in cellular architecture and division processes. We first monitored the localization of MIIP at centrioles in U2OS cells using regular immunofluorescence microscopy. Our results confirmed that MIIP is consistently associated with centrioles throughout the cell cycle both in interphase (Fig. 1b) and mitosis (Fig. 1c). Using U-ExM to further gain in resolution and by staining for tubulin as a proxy for the centriolar microtubule wall, we found that MIIP localizes to the proximal region of centrioles and corresponds thus to be a bona fide centriolar component (Fig. 1d)(40707486).