MYO5A

Cilia effects upon perturbation of MYO5A

Cilia number / % ciliated:

Decreased cilia number

Loss-of-function effect:

Shorter cilia

Ciliogenesis screen results (3 screens)

• Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-3.39) PMID:26167766
• Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
• Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:

abnormal behavior, abnormal cornea morphology, abnormal coat/hair pigmentation

Subcellular localization

basal body, cilia, cytosol, transition zone

Functional category

• Ciliary assembly/disassembly
• Trafficking (BBSome, small GTPases, vesicular transport, ATPases)
• Actin & cytoskeleton regulation
• Metabolism
• Viral interactions
• Signaling (Hedgehog, GPCRs, ion channels)
• Transition zone
• Transcription regulation

Function

Knockout results in reduction in ciliation, suggesting MYO5A is required for ciliogenesis. It might link microtubule cargo from Golgi or cilium (28710093). It mediates the transportation of preciliary vesicles to the mother centriole, depletion inhibits the attachment of preciliary vesicles to the distal appendages of the mother centriole and decreases cilia assembly (29335527). Interacts with the cilia- centrosomal protein RPGRIP1L (28266547).