NEDD1

NEDD1 gamma-tubulin ring complex targeting factor

Ensembl:
ENSG00000139350
UniProt:
Q8NHV4
OMIM:
600372
Synonyms:
GCP-WD, TUBGCP7

Cilia effects upon perturbation of NEDD1

Cilia number / % ciliated:
Decreased cilia number
Loss-of-function effect:
Shorter cilia

Ciliogenesis screen results (4 screens)

  • Kim2016: Not Reported
  • Wheway et al. 2015 (siRNA) [siRNA]: No effect PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381

Phenotypes

Mouse phenotype:
increased eosinophil cell number, preweaning lethality, complete penetrance, abnormal coat/ hair morphology, decreased circulating fructosamine level, embryonic lethality prior to organogenesis, increased circulating potassium level
Mouse ciliopathy phenotype:
decreased circulating calcium level

Subcellular localization

basal body, centrosome, cytosol

Functional category

  • Ciliary assembly/disassembly
  • Actin & cytoskeleton regulation
  • Protein processing & maturation

Function

Pk3 is required for 纬-tubulin and Nedd1 recruitment to the basal body, indicating a new function in basal body organization. This function of Pk3 may involve Wilms tumor protein 1-interacting protein (Wtip), which physically associates with and cooperates with Pk3 to regulate ciliogenesis.

Model organism evidence

Mus musculus (1 reference)

Strikingly, in cells with reduced Pk3 activity, cilia growth was inhibited and γ-tubulin and Nedd1 no longer associated with the basal body, suggesting that Pk3 has a novel function in basal body organization.

PMIDs: 27062996

Drosophila (1 reference)

Strikingly, in cells with reduced Pk3 activity, cilia growth was inhibited and γ-tubulin and Nedd1 no longer associated with the basal body, suggesting that Pk3 has a novel function in basal body organization.

PMIDs: 27062996

Xenopus (1 reference)

Strikingly, in cells with reduced Pk3 activity, cilia growth was inhibited and γ-tubulin and Nedd1 no longer associated with the basal body, suggesting that Pk3 has a novel function in basal body organization.

PMIDs: 27062996