NFAT5

nuclear factor of activated T cells 5

Tier: Gold Standard Ciliary Genes

Ensembl:
ENSG00000102908
UniProt:
O94916
OMIM:
604708
Synonyms:
KIAA0827, NF-AT5, NFATL1, NFATZ, OREBP, TONEBP

Cilia effects upon perturbation of NFAT5

Cilia number / % ciliated:
Decreased cilia number

Ciliogenesis screen results (6 screens)

  • Kim2016: Not Reported
  • Wheway et al. 2015 (siRNA) [siRNA]: Ciliogenesis Defect (z=-3.38) PMID:26167766
  • Breslow et al. 2018 (CRISPR) [CRISPR]: No Significant Effect PMID:29459680
  • Roosing et al. 2015 (siRNA) [siRNA]: No effect PMID:26595381
  • Pusapati et al. 2018 (CRISPR) [CRISPR]: Positive Regulator (Hh signaling, neg_rank=544, lfc=-2.53) PMID:30270045
  • Elliott et al. 2025 (CRISPRa) [CRISPRa]: Disassembly Trigger (casTLE Effect=-2.87) PMID:41160700

Phenotypes

Mouse phenotype:
preweaning lethality, incomplete penetrance, improved glucose tolerance, increased circulating chloride level, decreased hematocrit, increased circulating sodium level

Subcellular localization

basal body, nucleus

References: PMID:38961488

Functional category

  • Ciliary assembly/disassembly
  • Actin & cytoskeleton regulation
  • Cell migration & adhesion

Function

TonEBP controlled the expression and activity of aurora kinase A, TonEBP overexpression inhibited the loss of CP110 from the mother centrioles during the early stages of primary cilia assembly, and TonEBP regulated the localization of PCM1 and AZI1 (38961488).